Peru is one of the principal countries in mining extraction of minerals like gold, zinc, plumb and tin. Cyanide is used in gold production this compound is very toxic especially for environment, by nature reaction with sulfur it converts into thiocyanate (-SCN). In this paper, we isolate -SCN degrading fungus from Junin and Tumbes mining zones. Strains were assayed on 5 to 600 mM -SCN concentration, at least thiocyanate degrading kinetic was assayed at 1.2 g.L-1 of KSCN initial concentration, then the best strains had been morphological, biochemical and molecular identified using ITS RNA molecular marker. From 58 isolated fungus we found 4 unparfait funguses with a great thiocyanate degrading capacity those strains could be used for bioremediation processes. Thiocyanate degrading capacity of these strains was 50 g.L-1 on plate into 72 hours and their average capacity was 10.05 mg.L-1 -SCN with an standard velocity of -SCN degradation of 28.77 mg.L-1.h-1. Two strains were molecular identified as Fusarium trincictum by using ITS rDNA gene.