Weeds can be hosts of root-lesion nematode (Pratylenchus spp.), maintaining or increasing their population in the soil. The objective of this study was to evaluate the reaction of 25 weeds species to the nematode Pratylenchus zeae. The weed plants were individually inoculated with 1,000 individuals of P. zeae and maintained in a greenhouse for 90 days. After this period, eggs and nematodes were extracted, quantified, and the reproduction factor (RF = final population/initial population) was calculated. All tested weeds were susceptible (RF>1) to P. zeae, and the species Brachiaria decumbens, Rhynchelytrum repens, Digitaria insularis, D. horizontalis, B. brizantha were the most susceptible to this parasite.