The main unit of Mato Grosso do Sul vegetation is the Cerrado. For presenting an accelerated habitat loss process associated by low rate of flora knowledge, becomes of paramount importance the knowledge on tree species in this Biome. In this work, we to present a list of tree species of the Cerrado in Mato Grosso do Sul, in order to support future assessments of the state of conservation and implementation of proposals that can help to conserve its natural resources. The data were extracted from NeoTropTree, which consists of a database set of floristic lists of tree species. We recorded 541 tree species in 85 plant families and 252 genera, and three species are exclusive to the state.