Title
Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae
Date Issued
01 December 2019
Access level
metadata only access
Resource Type
journal article
Author(s)
YAURI CONDOR, KATHERINE SILVIA
Di Conza J.
GONZALES ESCALANTE, EDGAR
Gutkind G.
Publisher(s)
Elsevier B.V.
Abstract
A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presence of EDTA, in comparison with colistin alone. Results suggest that CPD-E may differentiate MCR-producing microorganisms from resistant microorganisms without this marker.
Volume
167
Language
English
OCDE Knowledge area
Otros temas de Biología
Biología del desarrollo
Scopus EID
2-s2.0-85074155276
PubMed ID
Source
Journal of Microbiological Methods
ISSN of the container
01677012
Sponsor(s)
E.G.E is a PhD student at UBA under a fellowship from the PRONABEC , Ministerio de Educación , Perú (RJ N° 142 – 2017 -MINEDU-VMGI-PRONABEC-OBPOST). GG and JDC are members of CONICET. Grateful acknowledgment is made to Nilton Lincopan, Rafael Vignoli and Rafael Cantón for their generous contribution with mcr-2-, mcr-4- and mcr-5-harboring strains.
E.G.E is a PhD student at UBA under a fellowship from the PRONABEC, Ministerio de Educaci?n, Per? (RJ N? 142 ? 2017-MINEDU-VMGI-PRONABEC-OBPOST). GG and JDC are members of CONICET. Grateful acknowledgment is made to Nilton Lincopan, Rafael Vignoli and Rafael Cant?n for their generous contribution with mcr-2-, mcr-4- and mcr-5-harboring strains.
Sources of information:
Directorio de Producción Científica
Scopus