Title
Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile
Date Issued
01 January 2015
Access level
open access
Resource Type
review
Author(s)
Kagohara L.T.
Schussel J.L.
Subbannayya T.
Sahasrabuddhe N.
Lebron C.
Brait M.
Maldonado L.
Valle B.L.
Pirini F.
Lopez J.
Letelier P.
Brebi-Mieville P.
Ili C.
Pandey A.
Chatterjee A.
Sidransky D.
Guerrero-Preston R.
Johns Hopkins University School of Medicine
Publisher(s)
Future Medicine Ltd.
Abstract
Aim: The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. Material & methods: DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Results: Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Conclusion: Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.
Start page
233
End page
249
Volume
11
Issue
2
Language
English
OCDE Knowledge area
Oncología
Genética, Herencia
Gastroenterología, Hepatología
Subjects
Scopus EID
2-s2.0-84921341357
PubMed ID
Source
Future Oncology
ISSN of the container
14796694
Sponsor(s)
National Cancer Institute - U01CA84986.
National Cancer Institute - K01CA164092.
Sources of information:
Directorio de Producción Científica
Scopus