Title
Neurite Tracing with Object Process
Date Issued
01 June 2016
Access level
open access
Resource Type
journal article
Author(s)
Basu S.
Ooi W.T.
Sorbonne Universités
Publisher(s)
Institute of Electrical and Electronics Engineers Inc.
Abstract
In this paper we present a pipeline for automatic analysis of neuronal morphology: from detection, modeling to digital reconstruction. First, we present an automatic, unsupervised object detection framework using stochastic marked point process. It extracts connected neuronal networks by fitting special configuration of marked objects to the centreline of the neurite branches in the image volume giving us position, local width and orientation information. Semantic modeling of neuronal morphology in terms of critical nodes like bifurcations and terminals, generates various geometric and morphology descriptors such as branching index, branching angles, total neurite length, internodal lengths for statistical inference on characteristic neuronal features. From the detected branches we reconstruct neuronal tree morphology using robust and efficient numerical fast marching methods. We capture a mathematical model abstracting out the relevant position, shape and connectivity information about neuronal branches from the microscopy data into connected minimum spanning trees. Such digital reconstruction is represented in standard SWC format, prevalent for archiving, sharing, and further analysis in the neuroimaging community. Our proposed pipeline outperforms state of the art methods in tracing accuracy and minimizes the subjective variability in reconstruction, inherent to semi-automatic methods.
Start page
1443
End page
1451
Volume
35
Issue
6
Language
English
OCDE Knowledge area
Radiología, Medicina nuclear, Imágenes médicas Bioinformática
Scopus EID
2-s2.0-84974722378
PubMed ID
Source
IEEE Transactions on Medical Imaging
ISSN of the container
02780062
Sources of information: Directorio de Producción Científica Scopus