Abarema auriculata crude extract EB689 showed cytotoxic activity against prostate, central nervous system and head-and-neck cancers, and may be useful in veterinary and human care in the future. For the first time, the presence of steroids and phenolic compounds were related to A. auriculata. Spinasterol, a bioactive steroid, was isolated from the non polar fractions of EB689, and the phenolic compounds neoastilbin, astilbin, isoastilbin, neoisoastilbin and engeletin were isolated from polar fractions of EB689. Preliminary toxicological results showed that EB689 exhibited a non-lethal dose of 4.90 mg/kg and a LD50 of 15.0 mg/kg. Higher doses of EB689 IP administered influenced general activity of male mice, provoking significant alterations in sensory system functions, including corneal reflex and tail squeeze and touch responses. Effects were also observed in psychomotor system function, particularly in hindquarter fall, surface-righting reflex, grasp reflex and body tone and in the autonomic nervous system, where piloerection, defecation, hypothermia, cyanosis and breathing were influenced. Significant alterations were observed in general activity, cyanosis and breath after the administration of the non-lethal dose (4.90 mg/kg), corroborating the initial observations. Diminishment of general activity and breathing and appearance of cyanosis are possibly related to intestine hemorrhage observed in necropsy, which led to animal death. The occurrence of the isolated compounds in EB 689 is unlikely related to animal death and it is the first time they are isolated from Abarema auriculata.