The problem of antibiotic resistance is considered a public and veterinary sanitary priority worldwide, for that reason the aim of the study was to evaluate the presence of antibiotic resistance against Salmonella spp., Escherichia coli coming from alpaca calves with and without diarrhea. The research was cross-sectional descriptive. 300 stool samples per rectal swab were collected from alpaca calves aging from 10 and 60 days with and without diarrhea from Peasant Communities in Huancavelica - Peru. The presence of Escherichia coli and Salmonella spp. was identified by conventional biochemical test, antibacterial susceptibility by Kirby Bauer method and 8 usual antibiotics from the veterinary market were evaluated. 100% of samples with diarrhea were positive to Escherichia coli; 40.0% Salmonella spp.; 40% Escherichia coli -Salmonella spp., and samples without diarrhea 48.3% positive to Escherichia coli; 14.0% Salmonella spp.; 9.3% Escherichia coli -Salmonella spp. The CMI in Escherichia coli and Salmonella spp. strains were resistant to Ampicillin (10.4 ± 0.3), (9.3 ± 0.2); Novomycin (11.1 ± 0.2), (11.2 ± 0. 1); Tetracycline (8.2 ± 0.1), (9.2 ± 0.3); Penicillin (9.1 ± 0. 4), (11.1 ± 0. 3); Gentamicin (10.1 ± 0. 4), (10.2 ± 0. 3) from samples with diarrhea and in samples without diarrhea resistant to Gentamicin (10.3 ± 0.1), (8.2 ± 0.1); Tetracycline (9.2 ± 0.4), (8.2 ± 0.4); Ampicillin (11.2 ± 0.1), (9.3 ± 0.2); Penicillin (10.2 ± 0.4), (10.1 ± 0.3). Salmonella spp., Escherichia coli strains isolated from alpaca calves with and without diarrhea show antibacterial resistance to multiple antibiotics used in veterinary.