Title
Fluorescent labeling reliably identifies Chlamydia trachomatis in living human endometrial cells and rapidly and accurately quantifies chlamydial inclusion forming units
Date Issued
01 December 2015
Access level
open access
Resource Type
journal article
Author(s)
The Ohio State University College of Medicine
Publisher(s)
Elsevier B.V.
Abstract
Chlamydia replication requires host lipid acquisition, allowing flow cytometry to identify Chlamydia-infected cells that accumulated fluorescent Golgi-specific lipid. Herein, we describe modifications to currently available methods that allow precise differentiation between uninfected and Chlamydia trachomatis-infected human endometrial cells and rapidly and accurately quantify chlamydial inclusion forming units.
Start page
79
End page
82
Volume
119
Language
English
OCDE Knowledge area
Biología celular, Microbiología
Ciencias socio biomédicas (planificación familiar, salud sexual, efectos políticos y sociales de la investigación biomédica)
Subjects
Scopus EID
2-s2.0-84944449967
PubMed ID
Source
Journal of Microbiological Methods
ISSN of the container
01677012
Sponsor(s)
National Institutes of Health - NIH
National Institute of Child Health and Human Development - R01HD072663 - NICHD
College of Medicine Office of Research, Ohio State University - COMOR
We thank Dr. Paul Stoodley and Dr. Luanne-Hall Stoodley for providing fluorescent microscopy support and Dr. Ann Thompson for thoughtful dialogue. The National Institute of Child and Human Development (grant R01HD072663 ) and The Ohio State University College of Medicine provided support for this work.
Sources of information:
Directorio de Producción Científica
Scopus