Title
Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
Date Issued
01 February 2012
Access level
metadata only access
Resource Type
journal article
Author(s)
Bustillo S.
Gay C.
García Denegri M.
Bal de Kier Joffé E.
Acosta O.
Leiva L.
Universidade Estadual de Campinas
Abstract
Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this invitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34μg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism. © 2011 Elsevier Ltd.
Start page
338
End page
343
Volume
59
Issue
2
Language
English
OCDE Knowledge area
Toxicología
Subjects
Scopus EID
2-s2.0-84855470663
PubMed ID
Source
Toxicon
ISSN of the container
18793150
Sponsor(s)
This research was financial supported by Secretaría General de Ciencia y Técnica ( PI B013-2010 ) and Agencia de Promoción Científica y Tecnológica. FonCyT. y SGCyT UNNE ( PICTO 2007-00143 ). The authors thank the Serpentarium of the local Zoo in Corrientes, Argentina for providing the venom from Bothrops alternatus species. Gay, C. and García Denegri. M. E. are recipients of research fellowship from Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET).
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas - CIEMAT
Secretaría General de Ciencia y Técnica, Universidad Nacional del Nordeste - PI B013-2010 - SGCyT, UNNE
Sources of information:
Directorio de Producción Científica
Scopus