In Ecuador, the City of Ambato has a public transport system which dependents exclusively on oil derivatives. The Directorate of Traffic, Transport and Mobility of the Decentralized Autonomous Government Municipality of Ambato, is responsible for the planning, organization and regulation of traffic of interparochial, and urban land transport throughout the territory comprising the jurisdiction of Ambato's Canton. This organism prepares and signs the contracts of operation for the public transport in the city of Ambato. In this paper, a comparative analysis of the energy consumption, economic costs and environmental impact between fossil and electric buses for the urban public transport of the city of Ambato is evaluated. This study is based on information provided by GADMA operating contracts of the Directorate of Traffic, Transport and Mobility on simulation of urban transport routes in ArcGIS software and on technical characteristic of fossil and electric buses provided by manufacturers. Innovation in issues of urban mobility and public transport is a new field of research to minimize the environmental impact caused by traditional systems. In this way, the impact of two brands of electric buses in terms of flexibility, sustainability and emission of pollutants is analyzed. The results show a remarkable reduction compared to the traditional system based on the aforementioned terms.