The Streptomyces are a group of actinobacteria considered as soil inhabitants sparsely studied in the field of plant biofertilization, and within the Peruvian economy Allium sativum L., (garlic) is one of the main horticultural crops in the Arequipa region. Therefore, we have proposed to characterize morphological, biochemical and genetic level, isolated rhizospheric actinobacteria of a wild area and examine their properties as plant growth promoting rhizobacteria (PGPR) in vitro and in vivo on seedlings of Allium sativum L. The in vitro analysis detected Streptomyces cirratus (ST3E) that fixed 0.63 ± 0.01 mg/L atmospheric nitrogen in the form of ammonium, Streptomyces sp (ST2A) was also detected as the best strain that synthesized 19.1 ± 0.82 μg/ml índole acetic índole acid, and all strains showed solubility indexes of inorganic phosphorus between 2.18 and 3.31. The in vivo test allowed to detect Streptomyces cirratus (ST3E), this has induced the increase of the size of the stem and increase of the dry weight and Streptomyces sp (ST2A), this increase the size of the root and the strains, Streptomyces sp (ST8A) and Streptomyces sp (ST8B), these have induced an increase in the diameter of the garlic bulb. Streptomyces cirratus (ST3E) is proposed as a candidate for deepend studies at molecular level as a possible fixer of atmospheric nitrogen. Strains of Streptomyces cirratus (ST3E), Streptomyces sp (ST2A), Streptomyces sp (ST8A) and Streptomyces sp (ST8B) are proposed as candidates for incorporation into bioformulates as biofertilization agents for the cultivation of garlic.