We introduce deepQuest-py, a framework for training and evaluation of large and lightweight models for Quality Estimation (QE). deepQuest-py provides access to (1) state-ofthe-art models based on pre-trained Transformers for sentence-level and word-level QE; (2) light-weight and efficient sentence-level models implemented via knowledge distillation; and (3) a web interface for testing models and visualising their predictions. deepQuest-py is available at https://github.com/sheffieldnlp/deepQuest-py under a CC BY-NC-SA licence.