The present study was aimed at isolating Argopecten purpuratus-associated actinomycetes strains, and evaluating their capacity of production of extracellular enzymes (ECE), as well as inhibitory compounds against relevant pathogens in commercial aquaculture. Twenty seven strains were isolated from the gut of A. purpuratus adult specimens that were collected in the city of Pisco and Pucusana (Lima) – Peru; 81.5 and 59.2% of the isolates showed proteolytic and lipolytic activity, respectively. In addition, the strains ARG 9-II, ARG 10-II, ARG 12-II and ARG14-II evidenced broad-spectrum antimicrobial activity against the pathogens Vibrio anguillarum, V. vulnificus, V. alginolitycus, Lactococcus garvieae, Streptococcus iniae, Carnobacterium piscícola, and Yersinia ruckeri. The strain ARG 4-II produced the highest inhibitory effect against S. iniae. The Minimum Inhibitory Concentration (MIC) of the dichloromethane extract obtained from the strain ARG 12-II supernatant was 250 and 500 μg ml-1 against V. anguillarum and V. alginolyticus, respectively. Based on 16S rRNA sequence analysis, strains ARG 9-II, ARG 12-II and ARG 14-II were identified as Streptomyces variabilis, St. labedae and St. rochei, respectively. Results indicate that A. purpuratus is a promissory source for the isolation of new strains of Streptomyces with high bioactivity and antagonistic capacity against relevant pathogens in aquaculture. These strains have high potential for being developed as probiotic agents in the future.