The purpose of this research work was to evaluate the effect of a mindfulness-based program on the academic stress levels of university students of Health Science. A multicenter, quasi-experimental, pretest and posttest study was conducted, with a sample of 116 students from a public university and a private university, divided at once into two control and experimental groups respectively. The SISCO inventory was used to measure the academic stress levels; which decreased in all experimental groups, after the intervention, finding statistical significance (p <0.05) in the results. Cohen's D scores were higher than 1.3, evidencing the effectiveness of the program.