Title
Datasets from harmonised metabolic phenotyping of root, tuber and banana crop
Date Issued
01 June 2022
Access level
open access
Resource Type
journal article
Author(s)
Drapal M.
Perez-Fons L.
Price E.J.
Amah D.
Bhattacharjee R.
Rouard M.
Swennen R.
Lopez-Lavalle L.A.B.
Fraser P.D.
Publisher(s)
Elsevier Inc.
Abstract
Biochemical characterisation of germplasm collections and crop wild relatives (CWRs) facilitates the assessment of biological potential and the selection of breeding lines for crop improvement. Data from the biochemical characterisation of staple root, tuber and banana (RTB) crops, i.e. banana (Musa spp.), cassava (Manihot esculenta), potato (Solanum tuberosum), sweet potato (Ipomoea batatas) and yam (Dioscorea spp.), using a metabolomics approach is presented. The data support the previously published research article “Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops” (Price et al., 2020) [1]. Diversity panels for each crop, which included a variety of species, accessions, landraces and CWRs, were characterised. The biochemical profile for potato was based on five elite lines under abiotic stress. Metabolites were extracted from the tissue of foliage and storage organs (tuber, root and banana pulp) via solvent partition. Extracts were analysed via a combination of liquid chromatography – mass spectrometry (LC-MS), gas chromatography (GC)-MS, high pressure liquid chromatography with photodiode array detector (HPLC-PDA) and ultra performance liquid chromatography (UPLC)-PDA. Metabolites were identified by mass spectral matching to in-house libraries comprised from authentic standards and comparison to databases or previously published literature.
Volume
42
Language
English
OCDE Knowledge area
Agricultura
Subjects
Scopus EID
2-s2.0-85126607674
Source
Data in Brief
ISSN of the container
23523409
Source funding
Bill and Melinda Gates Foundation
Sponsor(s)
The authors would like to thank International Potato Center (CIP, Peru), International Institute of Tropical Agriculture (IITA, Nigeria), International Center for Tropical Agriculture (CIAT, Colombia), Bioversity International Musa Germplasm Transit Centre (ITC, Belgium), Agricultural Research Centre for International Development (CIRAD, France) and Royal Botanical Gardens, Kew (United Kingdom), for providing in vitro plants and germplasm. This work was funded by the CGIAR Research Program on Roots, Tubers and Bananas (RTB) with support by CGIAR Fund Donors (www.cgiar.org/funders) and the African Cassava Whitefly Project (www.cassavawhitefly.org) through Natural Resources Institute (NRI), University of Greenwich, from a grant provided by the Bill and Melinda Gates Foundation (Grant OPP1058938).
This work was funded by the CGIAR Research Program on Roots, Tubers and Bananas (RTB) with support by CGIAR Fund Donors ( www.cgiar.org/funders ) and the African Cassava Whitefly Project ( www.cassavawhitefly.org ) through Natural Resources Institute (NRI), University of Greenwich, from a grant provided by the Bill and Melinda Gates Foundation (Grant OPP1058938).
Sources of information:
Directorio de Producción Científica
Scopus