Title
Deposition and imaging of metal-coated biomolecules with the STM
Date Issued
01 January 1992
Access level
metadata only access
Resource Type
journal article
Author(s)
Universidad de Oregon
Publisher(s)
Elsevier
Abstract
We have applied a simple and reliable procedure for imaging biomolecules with the scanning tunneling microscope (STM). The biomolecules are adsorbed on glow-discharged mica, then coated with a thin film of platinum-carbon. We have tested this method with linear and circular (plasmid) DNA molecules. The contrast and resolution of the STM images are comparable to electron micrographs of the same molecules when shadowes. Though the present lateral resolution (5-6 nm) is limited by the grain size of the conductive film, some details like supercoiled regions in the DNA are resolved. This method is interesting for two reasons. First, as an alternative technique for imaging biomolecules, Second, for use as a control in STM studies of bare biomolecules. © 1992.
Start page
1250
End page
1254
Volume
42-44
Issue
PART 2
Language
English
OCDE Knowledge area
BioquÃmica, BiologÃa molecular
Scopus EID
2-s2.0-0026898414
PubMed ID
Source
Ultramicroscopy
ISSN of the container
03043991
Sponsor(s)
National Institute of General Medical Sciences R01GM032543
Sources of information:
Directorio de Producción CientÃfica
Scopus