The identification of genes involved in cold adaptations of psychrotolerant bacteria Acidithiobacillus ferrivorans is important for biomining processes that take place at low temperatures like Andean mining installations in Peru. We have performed relative quantification RT-qPCR on candidate genes to have a role in adaptations at low temperature (5°C). The candidate genes analyzed were six: Two trehalose synthesis pathway genes, trehalose synthase (treS) and malto-oligosiltrehalose trehalohydrolase (treZ) showing no overexpression at 5°C. Two diguanylate cyclases genes related to exopolymer synthesis and biofilm formation (designated as dgc-I and dgc-II in this paper) were overexpressed at 21°C. The rusA and rusB genes involved in iron oxidation showed no significant change for rusA and no expression for rusB gene in any of both conditions. Genes rpoC, gyrB and alaS were validated as reference genes. These results show congruency with trancriptomics studies about gene expression of A. ferrivorans. Furthermore, the trehalose synthesis genes show no overexpression at low temperatures suggesting that other cold adaptation mechanisms are involved.