Title
Dehydration-induced physical strains of cellulose microfibrils in plant cell walls
Date Issued
01 October 2018
Access level
open access
Resource Type
journal article
Author(s)
Huang S.
Makarem M.
Kiemle S.
Zheng Y.
He X.
Ye D.
Gomez E.
Cosgrove D.
Kim S.
La Universidad Estatal de Pensilvania
Publisher(s)
Elsevier Ltd
Abstract
The effect of dehydration of plant cell walls on the physical status of cellulose microfibrils (CMFs) interspersed in pectin matrices was studied. Vibrational sum frequency generation (SFG) spectroscopy analysis of cellulose revealed reversible changes in spectral features upon dehydration and rehydration of onion epidermal walls used as a model primary cell wall (PCW). Combined with microscopic imaging and indentation modulus data, such changes could be attributed to local strains in CMFs due to the collapse of the pectin matrix upon dehydration. X-ray diffraction (XRD) showed that the (200) spacing of cellulose in dried PCWs is larger than that of cellulose Iβ obtained from tunicates. Thus, the modulus of CMFs in PCWs would be lower than those of highly-crystalline cellulose Iβ and inhomogeneous local bending or strain of CMFs could occur readily during the physical collapse of pectin matrix due to dehydration.
Start page
337
End page
348
Volume
197
Language
English
OCDE Knowledge area
Ciencia de los polímeros
Biología celular, Microbiología
Subjects
Scopus EID
2-s2.0-85048556844
PubMed ID
Source
Carbohydrate Polymers
ISSN of the container
01448617
Sponsor(s)
This work was supported by The Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award Number DE-SC0001090. The elastic modulus analysis with AFM was carried out with the support from National Science Foundation (Grant No. CMMI-1435766). We acknowledge Dr. Kabindra Kafle and Dr. Christopher M. Lee for the X-ray diffraction data of hardwood and cotton, respectively. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
This work was supported by The Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award Number DE-SC0001090 . The elastic modulus analysis with AFM was carried out with the support from National Science Foundation (Grant No. CMMI-1435766 ). We acknowledge Dr. Kabindra Kafle and Dr. Christopher M. Lee for the X-ray diffraction data of hardwood and cotton, respectively. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Sources of information:
Directorio de Producción Científica
Scopus