This study is aimed at determining the effects of dietary enzymolysis products of wheat gluten on the growth performance, organ weights, serum and intestinal antioxidant indices, serum biochemical indicators, and immunoglobulins of broiler chickens. The treatments consisted of a basal control diet, 2% of wheat gluten, and 1%, 2%, 3%, and 4% of enzymolysis products of wheat gluten. The inclusion of up to 4% of enzymolysis products of wheat gluten improved body weight and average daily gain. At 21 days, serum superoxide dismutase (T-SOD) had increased in the 2% of wheat gluten treatment (P <.05). At 42 days, both serum T-SOD and blood urea nitrogen had increased while uric acid had decreased (P <.05) in 2% enzymolysis products of wheat gluten treatment. These results showed that dietary inclusion of 2% enzymolysis products of wheat gluten can have beneficial effects on the growth performance and intestinal antioxidant activities of broiler chickens. Abbreviations: ADG: average daily gain; ADFI: average daily feed intake; AKP: alkaline phosphatase; BUN: blood urea nitrogen; BW: body weight; F/G: feed efficiency; GSH-Px: glutathione peroxidase; MDA: malondialdehyde; T-AOC: total antioxidant capacity; T-SOD: superoxide dismutase; CAT: catalase.