Background: Native fruits are gaining attention worldwide due to the wide range of macro- and micronutrients and phytochemicals that provide health benefits and impact diet-related non-communicable diseases. The objective of this study was to determine the glycemic index (GI) and glycemic load (GL) of native fruits. Methods: Twenty-six healthy volunteers (13 women and 13 men; ages 18-22 years) met the inclusion criteria for this study. Each participant ingested 50 g of carbohydrate equivalent with respect to fruits. Blood-glucose analysis by capillary puncture for glucose analysis were obtained at 15, 30, 45, 60, 90 and 120 minutes. Glycemic responses were evaluated by calculating the incremental area under the blood glucose curve. Results: The results indicated that the AUC for soursop was greater with respect to goldenberry, sachatomate and tumbo serrano. Regarding the GI values for the tested fruits, they varied between 24.36 to 36.19. Conclusions: The GI for the studied fruits were classified with low GI (≤55).