Introduction: Conjunctivitis is a common ocular infection in our environment. The indiscriminate use of broad spectrum antibiotics to treat conjunctivitis has generated resistant organisms. Objective: Identify the most common organisms isolated from samples of conjunctival origin and know their antibiotic susceptibility. Material and methods: Retrospective review of isolates from conjunctival scrapings obtained during 2011. Results: We collected 68 isolated of conjunctival origin. They were from 33 men (49%) and 35 women (51%). The average age was 47.23 years. The culture was positive in 75%, and we identified 11 microorganisms. The coagulase-negative Staphylococcus, led by Staphylococcus epidermidis were the most frequent microorganisms. The coagulase-negative Staphylococcus was sensitive to vancomycin, ciprofloxacin and gentamicin, and was resistant to cefazolin, sulfamethoxazole and oxacillin. 97% of isolates of Coagulase- negative Staphylococcus were multiresistant to antibiotics, being resistant to five or more antibiotics in most cases. Conclusions: Staphylococcus coagulase negative were the most frequent microorganisms isolated from conjunctival infections, most of the species isolated were susceptible to ciprofloxacin, gentamicin and vancomycin; most of the isolates showed multidrug resistance to antibiotics. ©2012 Sociedad Mexicana de Oftalmología.