Nonalcoholic fatty liver disease (NAFLD) is a prevalent disease in daily practice. Most General medical do not know how to make a proper diagnosis, treatment and prognosis of this disease. In addition, there were no studies, nor an instrument that measures the level of knowledge about NAFLD, so we found it necessary to conduct this research. To design and validate a questionnaire to determine the level of knowledge of nonalcoholic fatty liver disease (NAFLD) in general practitioners in the districts of Chicago and La Victoria since January to August 2011. We designed a questionnaire of 13 questions; we analyzed the content validity through expert assessment and construct validity using factor analysis. We assessed the reliability of the questionnaire examining the internal consistency by Cronbach's alpha method and test-retest reliability by intraclass correlation coefficient (ICC). The pilot test was conducted, interviewing 15 general medical. Cronbach's alpha of the questionnaire was 0.77. The value of Kaiser - Meyer-Olkin was 0.091 and Bartlett's sphericity test was 0.003, so we did not perform factor analysis, the intraclass correlation had a value of 77%. Also passing score for diagnosis was greater than 4.6 points and for treatment and prognosis than 4 points. This study provided an instrument with high reliability to measure the level of awareness of NAFLD in General Medical.