Solar disinfection (SODIS) of drinking water is a cost-effective household water treatment (HWT) method. Despite its simplicity of use, evidence suggest that SODIS water uptake remains lower than implementers planned. In this paper, we investigate socio-cultural factors associated with SODIS water uptake in rural Andean Bolivia. We conducted 28 semi-structured in-depth interviews and six focus group discussions within the framework of a community randomised trial to assess the uptake of SODIS water in the Bolivian Andes. Participants argued that SODIS does not produce an attractive type of drinking water despite acknowledging that SODIS represents a simple, safe, low-cost, and easy method of preparing safe drinking water. Attitudes towards SODIS water reflected local interpretations of water, health, and illness. The taste of SODIS water, the invisibility of water-borne pathogens, and habituation to untreated water represented the main barriers to SODIS water uptake. Alternative culturally adapted SODIS promotional campaigns (e.g., using herbs and additives to improve SODIS water taste) can increase its perceived desirability.