Objective: To increase the adherence of health care workers to three infections control practices through an educational intervention. Methods: 440 health care workers were trained in infection control during the period of 11 months. The methodology training Video-based Supplemental Instruction (VSI) affecting three infection control practices: Wash hands, N95 respirator use and prevention of needle stick accidents. They were evaluated 3 and 6 months post-intervention. Results: Obtained health care workers increased from 7% to 78% (p<0.05) in the proper hand washing technique, from 0% to 81.5% in the proper use of N95 respirator and was observed decrease in the proportion of sharp accidents in medical interns trained 0.20 vs. 0.44 (p<0.006). No difference was found in other populations intervened. Conclusion: The methodology VSI has positive effects on the adoption of infection control practices in health care workers.