Title
Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds
Date Issued
25 January 2016
Access level
metadata only access
Resource Type
journal article
Author(s)
Universidad de Santiago de Compostela
Publisher(s)
Elsevier
Abstract
Response surface methodology (RSM) was used to optimize the removal of five endocrine disrupting compounds (EDCs) by the enzyme versatile peroxidase (VP): bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). The optimal variables of enzyme activity (90-100UL-1), sodium malonate (29-43mM) and MnSO4 (0.8-1mM) led to very high removal rates of the five pollutants (2.5-5.0mgL-1min-1). The structural elucidation of transformation products arising from the enzymatic catalysis of the EDCs was investigated by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Liquid Chromatography Electrospray Time-of-Flight Mass Spectrometry (LC-ESI-TOF-MS). The presence of dimers and trimers, indicative of oxidative coupling, was demonstrated.
Start page
187
End page
195
Volume
33
Issue
1
Language
English
OCDE Knowledge area
Ingeniería química
Scopus EID
2-s2.0-84947019236
PubMed ID
Source
New Biotechnology
ISSN of the container
18716784
Source funding
Ministerio de Ciencia e Innovación
Sponsor(s)
This work was funded by the Spanish Ministry of Science and Innovation ( CTQ2013-44762-R ). The authors belong to the Galician Competitive Research Group GRC 2013-032 . G. Eibes thanks Xunta de Galicia for her postdoc grant through the I2C program.
Sources of information:
Directorio de Producción Científica
Scopus