Title
Culture isolation and molecular identification of blastocystis sp. In Brazilian human isolates: Preliminary results
Date Issued
01 January 2020
Access level
open access
Resource Type
journal article
Author(s)
de Melo G.B.
Malta F.d.M.
Lescano S.A.Z.
Castilho V.L.
Gonçalves E.M.D.N.
de Paula F.M.
Gryschek R.C.B.
Universidad de São Paulo
Publisher(s)
Instituto de Medicina Tropical de Sao Paulo
Abstract
Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova’s medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
Start page
1
End page
5
Volume
62
Language
English
OCDE Knowledge area
Parasitología
Tecnología médica de laboratorio (análisis de muestras, tecnologías para el diagnóstico)
Subjects
Scopus EID
2-s2.0-85088270442
PubMed ID
Source
Revista do Instituto de Medicina Tropical de Sao Paulo
ISSN of the container
00364665
Sponsor(s)
This study was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP grant Nº 2015/18213-6) and Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES grant Nº 1749729).
Sources of information:
Directorio de Producción Científica
Scopus