Title
Penicillinase-based amperometric biosensor for penicillin G
Date Issued
01 January 2014
Access level
open access
Resource Type
journal article
Author(s)
Universidade Estadual Paulista
Abstract
A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. © 2013 Elsevier B.V.
Start page
131
End page
133
Volume
38
Language
English
OCDE Knowledge area
Biotecnología industrial
Subjects
Scopus EID
2-s2.0-84889980313
Source
Electrochemistry Communications
ISSN of the container
13882481
Source funding
Fundação de Amparo à Pesquisa do Estado de São Paulo
Sponsor(s)
This work was supported by the São Paulo State Research Funding Agency (FAPESP) and São Paulo State University (UNESP) grants. WFAC acknowledges the CNPq (Brazilian National Research Council) for his MSc scholarship. LMG wishes to acknowledge the Portuguese Fundação para a Ciência e a Tecnologia (FCT) for his post-doctoral grant ( SFRH/BPD/76544/2011 ).
Sources of information:
Directorio de Producción Científica
Scopus