Evaluated on four non-target organisms: Daphnia magna, Chironomus calligraphus, Arthrospira platensis and Poecilia reticulata. In addition, the bioaccumulation of heavy metals was observed in the muscle tissue of three fish species (Oncorhynchus mykiss, Odonthestes bonariensis and Rhamdia quelen). It was found that for D. magna 25% of stations were toxic, for C. calligraphus, 100% of stations showed absence of toxic effects, for A. platensis, 50% of the stations were toxic and for P. reticulata the 8.3% of stations turned out to be toxic. The analysis of bioaccumulation in fish indicated the presence of high values in Hg and As.