Title
Imaging of metal-coated biological samples by scanning tunneling microscopy
Date Issued
02 May 1989
Access level
metadata only access
Resource Type
journal article
Author(s)
University of New Mexico
Abstract
A method for imaging biological samples by scanning tunneling microscopy (STM) is presented. There are two main difficulties in imaging biological samples by STM: (1) the low conductivity of biological material and (2) finding a method of reliably depositing the sample on a flat conducting surface. The first of these difficulties was solved by coating the samples with a thin film of platinum-carbon. The deposition problem was solved by a method similar to a procedure used to deposit biological molecules onto field ion microscope (FIM) tips. STM images of bacteriophage T7 and filamentous phage fd are shown. The substrate on which the samples were absorbed was atomically flat gold. The images do not show molecular detail due to the metal coating, but the gross dimensions and morphology are correct for each type of virus. Also, the surface density of virus particles increases and decreases in the way expected when the conditions of deposition are changed. These methods allow reliable and reproducible STM imaging of biological samples. © 1989.
Start page
367
End page
373
Volume
27
Issue
4
Language
English
OCDE Knowledge area
Bioquímica, Biología molecular
Biología celular, Microbiología
Scopus EID
2-s2.0-0024661833
PubMed ID
Source
Ultramicroscopy
ISSN of the container
03043991
Sponsor(s)
We would like to thank Arturo Baro for helpful discussions and suggestions, and Rebecca Keller and David Dunlap for help with some of the experiments. We also want to thank Carla Gray and Marcos Maestre for providing the samples of fd and T7, respectively. Support was provided by a grant from the National Institutes of Health (GM 32543 to C. Bustamante) and by National Science Foundation grants (DMG-8609654 and DMB-8501024 to C. Bustamante), the Center for High Technology Materials, UNM, and the office of the Vice-President of Research, UNM.
Sources of information:
Directorio de Producción Científica
Scopus