Currently, the use of clean technologies is being sought as a more natural and environmentally friendly strategy and alternative to reduce the concentration of carbon dioxide (CO2) in the environment in order to decrease the presence of this greenhouse gas that is harmful to the weakening of the ozone layer. One of these methods is the use of microalgae, which are photosynthetic microorganisms, capable of fixing this gas. The use of microalgae has advantages such as a minimum impact on the environment, it does not require a productive soil or clean water, from the microalgae is also generated biofuels and especially has the capacity to absorb CO2. The investigation had as purpose to determine the biocaptation of Carbon dioxide of the microalgae Scenedesmus sp. The method of test consisted of generating CO2 in the environment inside water with the alga in mention for a space of 12 days being able to reduce CO2, of an initial concentration of 0.002640 mg/L to 0.00556 mg/L, being corroborated that the microalgae Scenedesmus sp. is effective for biocaptation of Carbon dioxide. The cell density of the microalgae Scenedesmus sp. in the optimum treatment was of an initial number of cells of 1.200.000 cells/mL and ending with a total of 1.602.000 cells/mL with the temperature in the range of 22 °C to 27 °C, showing a stability and where there were no problems with the adaptation medium.