Title
Adherence of amino acids functionalized iron oxide nanoparticles on bacterial models E. Coli and B. subtilis
Date Issued
05 April 2018
Access level
open access
Resource Type
conference paper
Publisher(s)
Institute of Physics Publishing
Abstract
Magnetic iron oxides nanoparticles (NPs) functionalized with lysine (Lys) and arginine (Arg) was obtained by following chemical co-precipitation route in basic medium. The synthesis was performed by mixing ferrous chloride (FeCl2•4H2O), ferric chloride (FeCl3•6H2O) and the specific amino acid in a molar ratio of 1: 2: 0.5, respectively. High pH sample was washed several times with distilled water to reach a pH similar to distilled water (Ph=7) after the synthesis process, part of the NPs obtained was dried. Of the measurements of XRD and MS was obtained that the samples are magnetic nanoparticles of maghemite of about 9 nm in diameter. Of the FTIR and zeta potential measures was obtained that the amino acids Lys and Arg were correctly functionalized at magnetic nanoparticles, referred to herein as M@Lys and M@Arg. In order to demonstrate the capture and adhesion of the nanoparticles to the bacteria, scanning electron microscopy (SEM) was performed. The obtained visualization of both bacteria shows that they are coated by the magnetic particles. In addition, M@Lys (B. sutilis) were cultured to verify the inhibition of growth measured by colony forming units (CFU), the concentrations of M@Lys were 1.75x102 g/mL and 0.875x102 g/mL. After the confrontation obtained efficiencies of 75.63% and 98.75% respectively for the third dilution. While for the fourth dilution were 90% and 98.57% respectively were obtained for each concentration of nanoparticles. Hinting that a high efficiency of bacterial capture at very low concentrations of NPs, which gives us a tool to capture nanobiotechnology bacteria in liquid cultures with application to capture them in wastewater. Based on our results we concluded that NPS functionalized with the amino acids Lys and Arg adhere to the bacteria efficiently in low concentrations.
Volume
987
Issue
1
Language
English
OCDE Knowledge area
Ingeniería médica Nano-tecnología Física de partículas, Campos de la Física
Scopus EID
2-s2.0-85045617698
Source
Journal of Physics: Conference Series
ISSN of the container
1742-6588
Conference
Applied Nanotechnology and Nanoscience International Conference 2017, ANNIC 2017
Sources of information: Directorio de Producción Científica Scopus