Introduction: The conjunctiva is the tissue of the eye that gets infected with more frequency. The most common pathogens of conjunctivitis are viruses and bacteria. The indiscriminate use of broad-spectrum antibiotics to treat conjunctivitis generated resistant microorganisms. Objective: Identify the most common microorganisms isolated from samples of conjunctival origin and know their antibiotic susceptibility. Material and methods: Retrospective review of culture from conjunctival swabs obtained during 2012. Results: We collected 44 samples of conjunctival origin. They came from 21 males and 23 women. The median (25%, 75%) of age was 62 years (39-68). The culture was positive in 13 samples, identifying five different microorganisms. Staphylococcus epidermidis was the most common isolated microorganism (9 strains). All strains of S. epidermidis were sensitive to vancomycin, gentamicin, cefotaxime, ofloxacin and moxifloxacin. The multiple antibiotic resistance was identified in the majority of strains of S. epidermidis (6/9). Conclusions: Staphylococcus epidermidis was the most common isolated microorganism from samples of conjuntival infections. All strains of S. epidermidis were sensitive to vancomycin and moxifloxacin and most of them showed multidrug resistance to antibiotics.