Transgenic plants of the tropical forage legume Stylosanthes guianensis (Aubl.) Sw. CIAT 184 were regenerated from kanamycin- and phosphinotricin-resistant calli derived from Agrobacterium-inoculated leaf sections. Inoculations were carried out using the disarmed Agrobacterium tumefaciens strain EHA 101 harboring the binary vector pGV1040. pGV1040 T-DNA contains the bar and nptII genes as selectable markers, and the gusA gene as a screenable marker. Kanamycin was deleterious to plant regeneration, consequently phosphinotricin resistance was used for selection. Presence of the bar, npt II and gusA genes was detected during the regeneration process as well as in the transformed regenerated plants. Mendelian inheritance of the bar gene in the selfed progeny of the transformed plants was demonstrated. © 1994.