The aim this study was to relate the fetal and maternal serum biochemistry levels in the three thirds of the gestation period of the alpaca (Vicugna pacos). In total, 24 pregnant alpaca and their fetus were sampled to analyze levels of glucose (G), triglycerides (T), cholesterol (C), total protein (PT), albumin (A), calcium (Ca), total bilirubin (BT), direct bilirubin (BD), and alkaline phosphatase (FA). The number of animals in the first, second and third stage of gestation were 6, 7 and 11 respectively. The maternal-fetal correlations were G: 0.81, C: 0.70, T: 0.85, PT: 0.38, A: 0.38, FA: 0.29, Ca: 0.01, BT: 0.15 and BD: -0.05. The serum biochemistry levels were significantly different (from p<0.05) mainly in the third gestation period. It is concluded that all the biochemical parameters increased during gestation and the relationship between maternal-fetal serum biochemistry was positive.