In order to determine sleep habits in bus drivers and their relationship to accidents in the city of Arequipa, Peru, a crosssectional descriptive study was conducted in a non-probabilistic sample of 166 drivers. Driving hours per day were 9.4 ± 3.7. 54% (89) drive over 4 hours without stopping; 74% (123) drive at night; and 87% (145) sleep on the bus. 75% reported fatigue while driving (124). 27% (45) had drowsiness; 24% (40) reported having been in or on the verge of an accident while driving. Sleepiness or fatigue while driving was common in this population and their driving and rest habits could contribute to this.