Title
High Dimensional Single-Cell Analysis Reveals iNKT Cell Developmental Trajectories and Effector Fate Decision
Date Issued
08 September 2020
Access level
open access
Resource Type
journal article
Author(s)
Baranek T.
Lebrigand K.
Gonzalez L.
Bogard G.
Dietrich C.
Magnone V.
Boisseau C.
Jouan Y.
Trottein F.
Si-Tahar M.
Leite-de-Moraes M.
Mallevaey T.
Paget C.
University of Toronto
Publisher(s)
Elsevier B.V.
Abstract
Baranek et al. perform an unbiased single-cell transcriptomic analysis of thymic iNKT cells, which reveals an unappreciated transcriptional and functional heterogeneity among these innate T cells. Mathematical modelling and biological approaches yield a model that may reconcile the current conflicting models.
Volume
32
Issue
10
Language
English
OCDE Knowledge area
Biología celular, Microbiología
Scopus EID
2-s2.0-85090289674
PubMed ID
Source
Cell Reports
ISSN of the container
22111247
Sponsor(s)
This work was supported by the Agence Nationale de la Recherche “JCJC program” ( ANR-19-CE15-0032-01 ) and by the Region Centre-Val de Loire under the program “ARD 2020 Biomédicaments” ( Project 7UP ). M.S.-T. and C.P. are supported by Inserm . F.T. and M.L.-d.-M. are supported by CNRS . T.B., L.G., and C.B. are supported by the University of Tours . Roxane Lemoine (BCR Facility, University of Tours) and Julie Cazareth (CNRS, IPMC, Sophia-Antipolis) are acknowledged for their assistance with cell sorting. We acknowledge the UCAGenomiX platform, partner of the National Infrastructure France Génomique, supported by the Commissariat Aux Grands Investissements (ANR-10-INBS-09-03, ANR-10-INBS-09-02). Y. Wei (Institut Pasteur), M. Kronenberg (La Jolla Institute for Allergy & Immunology), J. Van Meerwijk (CPTP, Toulouse), and P. Fink (University of Washington) are acknowledged for the kind gift of Fhl2 −/− , Traj18 −/− , and Rag2 GFP mice, respectively. We also thank the University of Tours animal facility for excellent mouse husbandry and care. We thank the NIH tetramer core facility (Emory University) for providing CD1d tetramers. The graphical abstract was created using BioRender.com. K.L. designed and performed the bioinformatic analysis and analyzed data. C.d.A.H. G.B. C.D. L.G. C.B. V.M. and Y.J. performed the experiments. F.T. M.S. M.L.-d.-M. and T.M. provided key materials and critical input. T.B. and C.P. supervised the project, performed experiments, analyzed data, and wrote the paper with the input of all authors. The authors declare no competing interests.
Sources of information:
Directorio de Producción Científica
Scopus