Title
Cytotoxicity, genotoxicity and antibiofilm activity on Streptococcus mutans of an experimental self-etching adhesive system containing natural Butia capitata oil
Date Issued
01 October 2017
Access level
metadata only access
Resource Type
journal article
Author(s)
de Carvalho P.H.A.
de Pereira C.M.P.
Cury J.A.
Piva E.
Lund R.G.
Federal University of Pelotas
University of Campinas
Publisher(s)
Elsevier Ltd
Abstract
This study evaluated the biocompatibility and antibiofilm effects on Streptococcus mutans of an experimental self-etching adhesive system formulated with a natural vegetable product. The cytotoxicity of Clearfil Protect Bond (CPB), Clearfil SE Bond (CSEB), Adper SE Plus (AP), an experimental adhesive containing Butia capitata oil (EA) and an oil-free experimental adhesive as control (CA) was tested on 3T3/NIH mouse fibroblasts. Genotoxicity was indicated by micronuclei formation, and cell alterations were analyzed using light microscopy. To evaluate the effect of the adhesives on S. mutans biofilm, biomass samples were collected for dry weight and bacterial viability analyses, and the pH of the culture media was determined daily as an indicator of biofilm acidogenicity. The CA primer and EA uncured bond adhesive were the least toxic. No statistical difference was observed between EA and the untreated control, and EA showed similar effect to CPB. These findings suggest EA is biocompatible and presents activity against S. mutans biofilm.
Start page
95
End page
101
Volume
78
Language
English
OCDE Knowledge area
Otros temas de Biología
Biología celular, Microbiología
Subjects
Scopus EID
2-s2.0-85021710430
Source
International Journal of Adhesion and Adhesives
ISSN of the container
01437496
Sponsor(s)
This study received financial support from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [PROCAD-251/2007 and Edital CAPES/EMBRAPA n° 15/2014 (Proposal 158)], and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul – Edital PqG - Grant number #2162-2551/13-5).
Sources of information:
Directorio de Producción Científica
Scopus