Title
Canopy chlorophyll fluorescence applied to stress detection using an easy-to-build micro-lidar
Date Issued
01 October 2019
Access level
open access
Resource Type
Journal
Author(s)
International Potato Center
Abstract
LEDFLEX is a micro-lidar dedicated to the measurement of vegetation fluorescence. The light source consists of 4 blue Light-Emitting Diodes (LED) to illuminate part of the canopy in order to average the spatial variability of small crops. The fluorescence emitted in response to a 5-μs width pulse is separated from the ambient light through a synchronized detection. Both the reflectance and the fluorescence of the target are acquired simultaneously in exactly the same field of view, as well as the photosynthetic active radiation and air temperature. The footprint is about 1 m2 at a distance of 8 m. By increasing the number of LEDs longer ranges can be reached. The micro-lidar has been successfully applied under full sunlight conditions to establish the signature of water stress on pea (Pisum Sativum) canopy. Under well-watered conditions the diurnal cycle presents an M shape with a minimum (Fmin) at noon which is Fmin > Fo. After several days withholding watering, Fs decreases and Fmin < Fo. The same patterns were observed on mint (Menta Spicata) and sweet potatoes (Ipomoea batatas) canopies. Active fluorescence measurements with LEDFLEX produced robust fluorescence yield data as a result of the constancy of the excitation intensity and its geometry fixity. Passive methods based on Sun-Induced chlorophyll Fluorescence (SIF) that uses high-resolution spectrometers generate only flux data and are dependent on both the 3D structure of vegetation and variable irradiance conditions along the day. Parallel measurements with LEDFLEX should greatly improve the interpretation of SIF changes.
Start page
1
End page
15
Volume
142
Issue
1
OCDE Knowledge area
Química orgánica
Subjects
Scopus EID
2-s2.0-85067656123
PubMed ID
Source
Photosynthesis Research
Resource of which it is part
Photosynthesis Research
ISSN of the container
01668595
Source funding
Bill and Melinda Gates Foundation
Sources of information:
Directorio de Producción Científica
Scopus