Background and Objectives: It is common to use measures that do not directly assess the impact of anxiety on the health of people with HIV. The objective of the study was to evaluate the evidence of validity and reliability of the Spanish version of the Health-Related Anxiety Scale (HRAS). Materials and Methods: 100 patients diagnosed with HIV participated, selected by convenience sampling (76.5% men and 24.4% women; Average age = 39.96 years, SD = 13.19). The factorial structure of the HRAS was evaluated from the Confirmatory Factor Analysis (CFA). Likewise, the latent-level relationships of HRAS with life satisfaction, generalized anxiety, and depression were evaluated using a structural equation model. Reliability was assessed with McDonald’s omega coefficient