Title
Translation of Taenia crassiceps mRNA in cell-free heterologous systems
Date Issued
01 January 1978
Access level
metadata only access
Resource Type
journal article
Author(s)
Agosin M.
Department of Zoology, Riverbend Research Laboratories, University of Georgia, Athens, GA 30602, United States
Abstract
1. 1. mRNA isolated from larval Taenia crassiceps directs efficiently the synthesis of proteins in cell-free heterologous systems. 2. 2. Part of the newly synthesized proteins in a reticulocyte system are precipitable by a rabbit antiserum against T. crassiceps proteins. 3. 3. Analysis of the antiserum-protein dissociated complex by sodium dodecyl sulfate polyacrylamide gel electrophoresis indicates that most of the proteins synthesized are of low molecular weight (13,000-22,000) although a protein of mol. wt. 260,000 is also produced. 4. 4. Whether the newly synthesized proteins which are precipitable by specific antisera correspond to parasite antigens or to proteins with closely antigenic similarities remains to be established. © 1978.
Start page
183
End page
187
Volume
60
Issue
2
Language
English
OCDE Knowledge area
Genética, Herencia
Ciencias médicas, Ciencias de la salud
Scopus EID
2-s2.0-0017846177
PubMed ID
Source
Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN of the container
03050491
Sponsor(s)
Polysomes were isolated by a modification of the procedure described by Naquira et al. (1977). Homogenates * Supported in part by U.S. Public Health Service Research Grant, AI-09865-03, to M. Agosin. t To whom to address correspondence. :~ Present address: Instituto de Medicina Tropical, Universidad de San Marcos, Lima, Peru.
Sources of information:
Directorio de Producción Científica
Scopus