Title
Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region in Peru
Date Issued
01 January 1997
Access level
metadata only access
Resource Type
journal article
Author(s)
Ortega Y.
Roxas C.
Miller N.
Taquiri C.
Sterling C.
Johns Hopkins University
Publisher(s)
American Society of Tropical Medicine and Hygiene
Abstract
Cryptosporidium parvum and Cyclospora cayetanensis are protozoan pathogens that cause prolonged diarrhea in both immunocompetent and immunocompromised hosts. Cryptosporidium parvum can be transmitted via the fecal-oral route, while the exact mechanisms of transmission of Cyclospora cayetanensis have not been fully determined. Humans appear to be the sole host for the latter and a distinct seasonality has been observed in endemic areas around the world. Samples of vegetables were collected at several small markets in a periurban slum in Peru during the seasons of high and low incidence. The vegetables were washed, the supernatants were collected and centrifuged, and the pellets were resuspended in a solution of 2.5% potassium dichromate. Pellets were examined using direct microscopic observation, acid- fast staining, and immunofluorescent assays for C. parvum and Cyclospora cayetanensis oocysts. Samples were collected during three time periods: the season of low incidence, the beginning of the season of high incidence, and end of the season of high incidence. Of the total vegetables examined, 14.5% contained C. parvum oocysts and 1.8% had Cyclospora oocysts. Thus, market vegetables may provide a route by which Cryptosporidium and Cyclospora can be transmitted. Our study also suggests that washing vegetables does not completely remove Cryptosporidium and Cyclospora oocysts.
Start page
683
End page
686
Volume
57
Issue
6
Language
English
OCDE Knowledge area
Parasitología Agricultura Biología celular, Microbiología
Publication version
Version of Record
Scopus EID
2-s2.0-0031461059
PubMed ID
Source
American Journal of Tropical Medicine and Hygiene
ISSN of the container
0002-9637
Sponsor(s)
National Institute of Allergy and Infectious Diseases U01AI035894
Sources of information: Directorio de Producción Científica Scopus