Title
Validation of microbial source tracking markers for the attribution of fecal contamination in indoor-household environments of the Peruvian Amazon
Date Issued
15 November 2020
Access level
open access
Resource Type
journal article
Author(s)
Pisanic N.
Colston J.M.
Shapiama V.
Peñataro Yori P.
Heaney C.D.
Davis M.F.
University of Virginia
Johns Hopkins Bloomberg School of Medicine
Johns Hopkins Bloomberg School of Medicine
Publisher(s)
Elsevier B.V.
Abstract
The performance of eight microbial source tracking (MST) markers was evaluated in a low-resource, tropical community located in Iquitos, Peru. Fecal samples from humans, dogs, cats, rats, goats, buffalos, guinea-pigs, chickens, ducks, pigeons, and parrots were collected (n = 117). All samples were tested with human (BacHum, HF183-Taqman), dog (BactCan), pig (Pig-2-Bac), and avian (LA35, Av4143, ND5, cytB) markers using quantitative PCR (qPCR). Internal validity metrics were calculated using all animal fecal samples, as well as animal fecal samples contextually relevant for the Peruvian Amazon. Overall, Pig-2-Bac performed best, with 100% sensitivity and 88.5% specificity to detect the correct fecal source. Human-associated markers showed a sensitivity of 80.0% and 76.7%, and specificity of 66.2% and 67.6%. When limiting the analysis to contextually relevant animal fecal samples for the Peruvian Amazon, Av143 surpassed cytB with 95.7% sensitivity and 81.8% specificity. BactCan demonstrated 100% sensitivity and 47.4% specificity. The gene copy number detected by BacHum and HF183-Taqman were positively correlated (Pearson's correlation coefficient: 0.785), as well as avian markers cytB with Av4143 (Pearson's correlation coefficient: 0.508) and nd5 (Pearson's correlation coefficient: 0.949). These findings suggest that markers such as Av4143, Pig2Bac, cytb and BacHum have acceptable performance to be impactful in source attribution studies for zoonotic enteric disease transmission in this and similar low-resource communities.
Volume
743
Language
English
OCDE Knowledge area
Biología celular, Microbiología
Subjects
Scopus EID
2-s2.0-85088094027
PubMed ID
Source
Science of the Total Environment
ISSN of the container
00489697
Source funding
Source project
Sponsor(s)
F.S. was supported by FONDECYT-CONCYTEC (grant contract number 246-2015-FONDECYT ), and the National Institutes of Health Fogarty Global Health Fellows Consortium comprised of Johns Hopkins University , the University of North Carolina , Morehouse University , and Tulane University (grant no. D43TW009340 ). N·P was supported by a gift from the GRACE Communications Foundation, NIEHS grant R01ES026973 , and NIAID grants R21AI139784 and R43AI141265 . MNK was supported by the University of Virginia Departments of Infectious Diseases and Internal Medicine and the Bill & Melinda Gates Foundation ( OPP1066146 ). MFD was supported by the National Institutes of Health ( K01OD019918 to M.F.D.) and the Canine Health Foundation ( 02241 ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
F.S. was supported by FONDECYT-CONCYTEC (grant contract number 246-2015-FONDECYT), and the National Institutes of Health Fogarty Global Health Fellows Consortium comprised of Johns Hopkins University, the University of North Carolina, Morehouse University, and Tulane University (grant no. D43TW009340). N?P was supported by a gift from the GRACE Communications Foundation, NIEHS grant R01ES026973, and NIAID grants R21AI139784 and R43AI141265. MNK was supported by the University of Virginia Departments of Infectious Diseases and Internal Medicine and the Bill & Melinda Gates Foundation (OPP1066146). MFD was supported by the National Institutes of Health (K01OD019918 to M.F.D.) and the Canine Health Foundation (02241). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Sources of information:
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