We investigate the iridescence exhibited by Ceroglossus suturalis beetles, which mostly live endemically in the southern end of South America. Two differently colored specimens have been studied. We observed and characterized the samples by differentmicroscopy techniques, which revealed a multilayer structure within their cuticle. Using measured reflectance spectra as input data, we applied heuristic optimization techniques to estimate the refractive index values of the constituent materials, to be introduced within the theoretical model. The color of the samples was calculated for different incidence angles, showing that multilayer interference is the mechanism responsible for the observed iridescence. © 2013 Optical Society of America.