Asparagus is one of the most important export crops for Peru. In the two main production zones: Ica and Libertad, asparagus fields produce over 12 years before yield declining is observed. The interaction between Fusarium oxysporum f.sp. asparagi (FOA) and Meloidogyne incognita (MI) is the most limiting factor for replanting asparagus fields in Peru. This experiment was conducted at La Molina, Lima. Soil samples from a 10 years old asparagus field from the Chavimochic area, La Libertad, were collected. Ten asparagus cultivars (‘UC157’, ‘UC115’, ‘Atlas', ‘Cutlas’, ‘Espada’, ‘NJ1113’, ‘NJ1025’, ‘FCE1*M256’, ‘2H’, ‘38H’) were planted in pots with soil collected from replanted sites or sand and raised under greenhouse conditions. FOA symptoms were evaluated in the main and secondary asparagus roots in terms of lesions, root mass loss, damage index, root length, fern and root fresh and dry weight, dry matter content and dry matter ratio between fern and root, MI nodulation (Zeck’s scale) and MI final and initial population ratio (Pf/Pi). Fusarium oxysporum f.sp. asparagi and Meloidogyne incognita were found in all cultivars root system. ‘UC157’ and ‘UC115’ were the most susceptible. ‘Atlas’ and ‘FCE1*M256’ were the least susceptible since both showed the lowest values in all parameters evaluated in their roots.