The sediments of Lake Titicaca contain heavy metals of mining origin, suggesting the contamination of the fish farms that develop in its waters. The present study aimed to evaluate the concentration of heavy metals in the muscles of rainbow trout (Oncorhynchus mykiss) raised in the northeast area of Lake Titicaca, at an altitude of 3812 m, located in the provinces of Puno and Huancané, Peru. The heavy metals present in the trout meat were analyzed through the spectrophotometric method using the inductively coupled plasma technique together with an ICP-OES optical emission spectrophotometer. The sequence of the concentration of heavy metals found in the muscles of the trout collected from the province of Puno was Zn> Fe> Cd> Mn> Pb> Cu> Hg and in the province of Huancané was Zn> Fe> Mn> Cu> Pb> Cd> Hg. The concentrations found did not exceed the maximum permissible limits recommended by WHO/FAO, so the trout from the evaluated areas are suitable for human consumption.