Objective: To determine the prevalence of musculoskeletal disorders in health workers at the first level of care. Material and Methods: Transversal descriptive study, involving 278 workers of both sexes who provide health services in the health centers of the first level of care located in the Callao Region. The instrument used was the Kuorinka Nordic Questionnaire. Results: Of the sample, 69.4% (n = 193) were women and the rest was composed of men. Of the participants, 34.5% (n = 96) were nursing professionals, 28.8% (n = 80) were nursing technicians and the rest consisted of doctors, obstetricians and dentists. In the last 12 months, pain, discomfort or discomfort were mainly in the cervical, dorsal and lumbar region, with 46% (n = 128), 34.5% (n = 96) and 28.1% (n = 78), respectively, whereas in the last 7 days they were mainly in the cervical, lumbar and dorsal region, present in 17.6% (n = 49), 13.3% (n = 37) and 11.9% (n = 33) respectively. Pain, discomfort or discomfort in the lumbar region, during the last 12 months and in the last 7 days, has mainly been presented in females (p <0.05). Conclusions: The musculoskeletal disorders among workers of the first level of care are frequent, affects especially the lower back, mainly the female sex, and nursing technicians, generating limitations for work performance. It is recommended to take preventive measures in workers of the first level of care.