The objective of this study was to evaluate the biosorption dynamics of heavy metals by the dead macrophyte Egeria densa in fixed bed column. Experiments were conducted with mono- and bi-component solutions of Cd(II) and Zn(II) at pH 4.0, temperature of 30 °C and volumetric flow rate range between 2 and 6 cm3 min-1. A mass transfer mathematical model was applied for describing the mono-component biosorption dynamics. In this model, the equilibrium was represented by the batch Langmuir isotherm, and intraparticle diffusion was considered as the rate-limiting step for the mass transfer. The mass transfer coefficient (KS) was estimated by the downhill simplex optimization method for each volumetric flow tested, while the axial dispersion coefficient was calculated by empirical correlations. The KS coefficients and the equilibrium parameters obtained in a mono-component batch system were validated using data from the breakthrough curves of the Cd(II) and Zn(II) binary mixture obtained for different volumetric flows and initial concentrations of each metal ion.