This is the first study to compare plasma depletion drifts with the ambient plasma drifts and neutral winds in the post sunset equatorial ionosphere using global-scale satellite observations. The local time and latitude variations of the drift velocities of O+ plasma depletions at 350-400 km altitude are derived from the observations of the far ultraviolet imager operated on the IMAGE satellite during 10 March to 7 June 2002. These depletion drift velocities are compared with the simultaneously measured ion drift velocities and neutral winds by the ROCSAT-1 and the CHAMP satellites for a similar time period. The analysis shows that the zonal drift velocity of plasma depletions is smaller than both the ambient ion zonal drift velocity and the neutral zonal wind at 18:00-20:00 magnetic local time, and after 21:00, the variations of these velocities are similar. The difference of the plasma depletion drift with the background is found to be smaller at lower latitudes. Furthermore, the zonal drift velocity of the depletion is found to have a large latitudinal gradient specifically at 12°-18° magnetic latitude, which again does not match the ambient ion drift and the neutral wind. This latitudinal difference has been reported by previous studies, but those studies use models and they only compare the depletion drifts with the modeled neutral winds. This study provides a measure of the difference that has never been studied before by any study using global observations. It has been suggested that polarization electric fields inside the plasma depletion structure drive the plasma to drift westward and thus the depletion structure moves to the east. The latitudinal gradient of the depletion drift velocity seen here in this study could also be explained by the polarization electric fields. For the C-shaped (reversed C) depletion, the polarization electric fields inside the depletion drive a westward drift of plasma and this drift velocity changes with increasing latitude. Consequently, the depletion drift has a latitudinal gradient becoming significant at higher latitudes. Key Points First comparison of depletion drifts with ion drifts and winds using satellites Difference of plasma depletion drift velocity with background wind at 18-20h LT. Difference of latitudinal gradient in depletion drift and ion drift ©2013. American Geophysical Union. All Rights Reserved.