Producing large quantities of high quality mini-tubers at low cost is essential for an economically viable supply of seed potatoes. Here we systematize the technical and economic aspects of aeroponics and provide a benchmark comparison of this technology with other mini-tuber production systems as developed in Latin America: conventional, semi-hydroponics, and fiber-cement tiles technology. Research methodologies included: 3-year registration of cash flows and production registers of aeroponics, economic and technical surveys, in-depth inquiry with managers of technologies. Results show that aeroponics as promoted by the International Potato Center (CIP) has several advantages, including high multiplication rates (up to 1:45), high production efficiency per area (> 900 mini-tubers per m2), savings in water, chemicals and/or energy, and positive economic indicators. The fiber-cement tiles technology from Brazil is also shown to be highly efficient and slightly more robust compared to aeroponics. Preconditions for the successful adoption of the different technologies in developing countries are discussed. © 2013 Potato Association of America.